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Human Interleukin 33 (rHuIL-33) Human Interleukin 33 (rHuIL-33)

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Human Interleukin 33 (rHuIL-33)

Strength:
Availability:
Quantity:
 
 
  • SJB15

  • 10μg, 100μg

Specifications and Use

Specifications and Use

Description:

Recombinant human interleukin 33 is a single, non-glycosylated polypeptide chain containing 159 amino acids, none disulfide bonds and having a molecular mass of approximately 18.0 kD.

Source: E. coli.

Molecular Mass: Approximately 18.0kD.

Purity: ≥97%.

Endotoxin Level: <1EU/µg, determined by the LAL method.

Biological Activity:

Measured in a cell proliferation assay using D10.G4.1, ED50: 0.3ng/ml -3ng/ml.

Formulation:

Lyophilized from a 0.2µm filtered solution in 1mM DTT, 1mM EDTA, 0.1% HSA, PBS pH6.5.

Reconstitution:

It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.  

Storage:

  • Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.

  • Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

  • Avoid repeated freeze-thaw cycles.


For Laboratory Use Only.


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