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Human Interleukin 21 (rHuIL-21) Human Interleukin 21 (rHuIL-21)

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Human Interleukin 21 (rHuIL-21)

Strength:
Availability:
Quantity:
 
 
  • SJB14

  • 10μg, 100μg

Specifications and Use

Description:

Recombinant human IL-21 is a single, non-glycosylated, polypeptide chain containing 133 amino acids, two pairs of disulfide bonds.

Source: E. coli.

Molecular Mass: Approximately 15.5kD.

Purity: ≥97%.

Endotoxin Level: <1EU/µg, determined by the LAL method.

Biological Activity:

Measured in a cell proliferation assay using B9-11 cells. The ED50 is around 50-350ng/ml.

Formulation:

Lyophilized from a 0.2μm filtered solution in PBS containing 0.1% HSA, pH7.4.

Reconstitution:

It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.

Storage:

  • Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.

  • Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

  • Avoid repeated freeze-thaw cycles.


For Laboratory Use Only.


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