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Human GM-CSF (rHuGM-CSF) Human GM-CSF (rHuGM-CSF)

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Human GM-CSF (rHuGM-CSF)

Strength:
Availability:
Quantity:
  • SJA01

  • 10µg, 50µg

Specifications and Use

Description:

Recombinant human GM-CSF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.6kD.

Source: E. coli.

Molecular Mass: Approximately 14.6kD.

Purity: ≥97%.

Endotoxin Level: <1EU/µg, determined by the LAL method.

Biological Activity:

Measured in a cell proliferation assay using the factor-dependent cell line, TF-1, the specific activity shall be not less than 1×107IU/mg.

Formulation:

Lyophilized from a 0.2μm filtered solution in 10mM Phosphate Buffer.

Reconstitution:

It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.

Storage:

  • Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.

  • Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70 in a manual defrost freezer for three months without detectable loss of activity.

  • Avoid repeated freeze-thaw cycles.


For Laboratory Use Only.


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